DNA

Part:BBa_K2308004

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-04)


used to knock out the gene crtB in the genome of Rhodobacter Sphaeroides+ Amp

This part is used to knock out the gene crtB in Rhodobacter Sphaeroides, using the method of redET. The part is composed of 4 basic parts(BBa_K2308006,BBa_K2308010,BBa_K2308011,BBa_K2308007) If the gene crtB is knocked out by homologous recombination, the strain will be selected by Amp resistance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 47
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1250
    Illegal BsaI.rc site found at 162
    Illegal SapI site found at 1759


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Categories
Parameters
None